7 research outputs found
Active mechanics reveal molecular-scale force kinetics in living oocytes
Active diffusion of intracellular components is emerging as an important
process in cell biology. This process is mediated by complex assemblies of
molecular motors and cytoskeletal filaments that drive force generation in the
cytoplasm and facilitate enhanced motion. The kinetics of molecular motors have
been precisely characterized in-vitro by single molecule approaches, however,
their in-vivo behavior remains elusive. Here, we study the active diffusion of
vesicles in mouse oocytes, where this process plays a key role in nuclear
positioning during development, and combine an experimental and theoretical
framework to extract molecular-scale force kinetics (force, power-stroke, and
velocity) of the in-vivo active process. Assuming a single dominant process, we
find that the nonequilibrium activity induces rapid kicks of duration 300 s resulting in an average force of 0.4 pN on vesicles
in in-vivo oocytes, remarkably similar to the kinetics of in-vitro myosin-V.
Our results reveal that measuring in-vivo active fluctuations allows extraction
of the molecular-scale activity in agreement with single-molecule studies and
demonstrates a mesoscopic framework to access force kinetics.Comment: 20 pages, 4 figures, see ancillary files for Supplementary Materials,
* equally contributing author
Jupyter Notebooks – a publishing format for reproducible computational workflows
It is increasingly necessary for researchers in all fields to write computer code, and in order to reproduce research results, it is important that this code is published. We present Jupyter notebooks, a document format for publishing code, results and explanations in a form that is both readable and executable. We discuss various tools and use cases for notebook documents
Papyri: better documentation for the scientific ecosystem in Jupyter
International audienceWe present here the idea behind Papyri, a framework we are developing to provide a better documentation experience for the scientific ecosystem. In particular, we wish to provide a documentation browser (from within Jupyter or other IDEs and Python editors) that gives a unified experience, cross library navigation search and indexing. By decoupling documentation generation from rendering we hope this can help address some of the documentation accessibility concerns, and allow customisation based on users' preferences
Active diffusion positions the nucleus in mouse oocytes
International audienc
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